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Image Search Results
Journal: PLoS ONE
Article Title: Characterization of the SUMO-Binding Activity of the Myeloproliferative and Mental Retardation (MYM)-Type Zinc Fingers in ZNF261 and ZNF198
doi: 10.1371/journal.pone.0105271
Figure Lengend Snippet: A: U2OS cells were transfected with constructs encoding myc-tagged ZNF198 full-length or truncation fragments. Localization of ZNF198 with PML-NBs was analyzed by fluorescence microscopy. B : Full-length and truncation fragments of myc-tagged ZNF198 were expressed in U2OS cells, immunopurified with anti-myc agarose beads, and detected with anti-myc and anti-SUMO-2 antibodies. Full-length protein bands are marked with an asterisk in each input lane. C : Myc-tagged ZNF198 and FLAG-tagged PML were co-expressed in U2OS cells, immunopurified with anti-myc agarose beads or anti-FLAG agarose beads, and detected with anti-myc and anti-PML antibodies.
Article Snippet: GST, GST-tagged SUMO-1, SUMO-2, SUMO-2(x3), and SUMO-2(QFI) were purified by affinity chromatography on
Techniques: Transfection, Construct, Fluorescence, Microscopy